期刊
ELECTROANALYSIS
卷 19, 期 17, 页码 1831-1839出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200703943
关键词
electrochemical immunoassay; gold hollow microspheres; carbohydrate antigen-125; porous polythionine
A new electrochemical immunosensor for the detection of carbohydrate antigen-125 (CA125), a carcinoma antigen, was developed by immobilization CA125 antibody (anti-CA125) on gold hollow microspheres and porous polythionine (PTH) modified glassy carbon electrodes (GCE). The gold hollow microspheres provided a biocompatible microenvironment for proteins, and greatly amplified the coverage of anti-CA125 molecules on the electrode surface. The performance and factors influencing the immunosensor were investigated in detail. The detection is based on the current change before and after the antigen-antibody interaction. Under optimal conditions, the amperometric changes were proportional to CA125 concentration ranging from 4.5 to 36.5 U/mL with a detection limit of 1.3 U/mL (at 3 sigma). The CA125 immunosensor exhibited good precision, high sensitivity, acceptable stability, accuracy and reproducibility. The as-prepared immunosensors were used to analyze CA125 in human serum specimens. Analytical results suggest that the developed immunoassay has a promising alternative approach for detecting CA125 in the clinical diagnosis.
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