期刊
ANALYTICA CHIMICA ACTA
卷 633, 期 1, 页码 103-108出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2008.11.035
关键词
Suspension array; Multiplex assay; Tumor marker detection; Immunoassay; Colloidal crystal bead
资金
- Ministry of Science and Technology [2006AA03Z331]
- National Science Foundation of China [20573018, 60121101, 20703010]
A novel photonic suspension array was developed for multiplex immunoassay. The carries of this array were silica colloidal crystal beads (SCCBs). The codes of these carriers are the characteristic reflection peak originated from their structural periodicity, and therefore they do not suffer from fading, bleaching, quenching, and chemical instability. In addition. because no dyes or materials related with fluorescence are included, the fluorescence background of SCCBs is very low. With a sandwich format, the proposed suspension array was used for Simultaneous multiplex detection of tumor markers in one test tube. The results showed that the four tumor markets, alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), carcinoma antigen 125 (CA 125) and carcinoma antigen 19-9 (CA 19-9) could be assayed in the ranges of 1.0-500 ng mL(-1), 1.0-500 ng mL(-1), 1.0-500 U mL(-1) and 3.0-500 U mL(-1) with limits of detection of 0.68 ng mL(-1), 0.95 ng mL(-1), 0.99 U mL(-1) and 2.30 U mL(-1) at 3 sigma, respectively. The proposed array showed acceptable accuracy, detection reproducibility, storage stability and the results obtained were in acceptable agreement with those from parallel single-analyte test of practical clinical sera. This technique provides a new strategy for low Cost, automated, and Simultaneous multiplex immunoassay. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据