期刊
ANALYTICA CHIMICA ACTA
卷 634, 期 1, 页码 54-60出版社
ELSEVIER
DOI: 10.1016/j.aca.2008.12.012
关键词
Solid phase extraction; 96-Well plate; Phenols; Folin-Ciocalteu; Biomarker; Urine; PREDIMED; Polyphenols stability
资金
- CICYT's [AGL2005-002823, 2006-14228]
- Red de Grupo [G03/140]
- Spanish Ministries of Education and Science and of Health [PI 052443, PI 070240]
- RETICS [RD06/0045/0003]
- Instituto de Salud Carlos III
- INGENIO-CONSOLIDER [CSD2007-063]
- Spanish Ministry of Education and Science
- Generalitat of Catalonia
- University of Barcelona
Nutritional markers have several advantages for epidemiologic and clinical assays, when compared to dietary data obtained by food frequency questionnaires. Few studies have assessed whether total polyphenol (TP) compounds provide a valid biomarker for TP intake. To date, there has been almost no literature describing methods to determine TP in complex matrices such as urine, which have many interfering substances. We report a rapid Folin-Ciocalteu method to determine TP in urine samples using Oasis (R) MAX 96-well plate cartridges for solid phase extraction. These plates allow analysis of a high number of samples at the same time. We performed a prospective, randomized, crossover trial and one cross-sectional study with 60 volunteers from the PREDIMED trial, seeking to evaluate whether the TP in urine were correlated with polyphenol intake and Could, therefore, be considered as a market of intake of these compounds. The assay was optimized; the sensitivity and the polarity range of urine polyphenols were increased and the detection and quantification limits were significantly reduced. The metabolites in standards solution and urine samples were stable under the storage and handling conditions. In the clinical trial and the cross-sectional study,TP excreted in Spot urine samples were positively correlated with TP intake, r=0.48, P<0.01 and r=0.257, P=0.04. respectively. The methodology described may be used to detect TP in urine samples, employing the high throughput of 96-well microtiter plates and reader. The method is fast and simple and it allows analysis of a large number of samples at the same time. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据