期刊
ANALYTICA CHIMICA ACTA
卷 647, 期 2, 页码 125-136出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2009.06.003
关键词
Food contaminants; Hapten design; Immunoassay; Molecular modeling
资金
- National High Technology Research and Development Program of China [2006AA10Z44, 2007AA10Z437, 2006BAD27B02-05]
- National Natural Science Foundation of China [30700663, 20877029]
- Natural Science Foundation of Guangdong Province [06300421]
Immunoassay for low molecular weight food contaminants, such as pesticides, veterinary drugs, and mycotoxins is now a well-established technique which meets the demand for a rapid, reliable, and cost-effective analytical method. However, due to limited understanding of the molecular structure of antibody binding sites and antigenic epitopes, as well as the intermolecular binding forces that come into play, the traditional 'trial and error' method used to develop antibodies still remains the method of choice. Therefore, development of enhanced immunochemical techniques for specific- and generic-assays, requires new approaches for antibody design that will improve affinity and specificity of the antibody in a more rapid and economic manner. Computer-assisted molecular modeling (CAMM) has been demonstrated to be a useful tool to help the immunochemist develop immunoassays. CAMM methods can be used to help direct improvements to important antibody features, and can provide insights into the effects of molecular structure on biological activity that are difficult or impossible to obtain in any other way. In this review, we briefly summarize applications of CAMM in immunoassay development, including assisting in hapten design, explaining cross-reactivity, modeling antibody-antigen interactions, and providing insights into the effects of the mouse body temperature on the three-dimensional conformation of a hapten during antibody production. The fundamentals and theory, programs and software, limitations, and prospects of CAMM in immunoassay development were also discussed. (C) 2009 Elsevier B.V. All rights reserved.
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