期刊
ANALYTICA CHIMICA ACTA
卷 649, 期 2, 页码 230-235出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2009.07.039
关键词
Carcinogenic; Alkaloid; Arecoline; Areca nut; Small molecular weight; Matrix-assisted laser desorption ionization; Imaging
资金
- National Science Council [NSC 96-2113 M037-015, NSC 97-2113-M-037-009, NSC 96-2113-M-037-016, 97-2113-M-037-008-MY2]
Arecoline is the main alkaloid present in the areca nut (or betel nut) and it has central nervous system effects. its pharmacological activities induce the constriction of the bronchial smooth muscles, and stimulation of the lacrimal and intestinal glands. Chewing areca nut is harmful to health because this habit may increase the risk of the development of oral cancer. In this study, a fast method was provided for the determination of areca alkaloids by matrix-assisted laser desorption ionization (MALDI) mass spectrometer with a time-of-flight (TOF) analyzer. Traditionally the MALDI-TOF method was not suitable for the analysis of small molecular weight (m/z < 600) compounds because of the high background of the matrix. In this study, a new matrix was utilized to decrease the background interference effectively. After simple sample preparation. 1 mu L sample supernatant was mixed with 1 mu L matrix and then deposited on the target plate. This new matrix was also used to test the MALDI imaging experiment. Application of this MALDI-TOF method for trace analysis of arecoline by this new matrix in human plasma at sub mu M level proved workable. (C) 2009 Elsevier B.V. All rights reserved.
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