期刊
DEVELOPMENTAL CELL
卷 13, 期 3, 页码 391-404出版社
CELL PRESS
DOI: 10.1016/j.devcel.2007.07.007
关键词
-
资金
- NIDDK NIH HHS [DK061618, DK076956] Funding Source: Medline
Insulin stimulates glucose transport in muscle and adipose tissue by producing translocation of the glucose transporter Glut4. The exocyst, an evolutionarily conserved vesicle tethering complex, is crucial for targeting Glut4 to the plasma membrane. Here we report that insulin regulates this process via the G protein RaIA, which is present in Glut4 vesicles and interacts with the exocyst in adipocytes. Insulin stimulates the activity of RaIA in a PI 3-kinase-dependent manner. Disruption of RaIA function by dominant-negative mutants or siRNA-mediated knockdown attenuates insulin-stimulated glucose transport. RaIA also interacts with Myo1c, a molecular motor implicated in Glut4 trafficking. This interaction is modulated by Calmodulin, which functions as the light chain for Myo1c during insulin-stimulated glucose uptake. Thus, RaIA serves two functions in insulin action: as a cargo receptor for the Myo1c motor, and as a signal for the unification of the exocyst to target Glut4 vesicles to the plasma membrane.
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