期刊
ANALYTICA CHIMICA ACTA
卷 635, 期 2, 页码 188-195出版社
ELSEVIER
DOI: 10.1016/j.aca.2009.01.021
关键词
Oleanolic acid; Ursolic acid; NMR; Mixture analysis; H-1-C-13 heteronuclear single-quantum coherence; H-1-C-13 heteranuclear multiple-bond correlation
资金
- European Union-European Social Fund (ESF)
- Hellenic Ministry of Education
- Greek Community Support Framework III, Regional Operational Program of Epirus [MIS 91629]
- NMR Center of the University of Ioannina
A novel strategy for NMR analysis of mixtures of oleanolic and ursolic acids that occur in natural products is described. These important phytochemicals have similar structure and their discrimination and quantification is rather difficult. We report herein the combined use of proton-carbon heteronuclear single-quantum coherence (H-1-C-13 HSQC) and proton-carbon heteronuclear multiple-bond correlation (H-1-C-13 HMBC) NMR spectroscopy, in the identification and quantitation of oleanolic acid (CA) and ursolic acid (UA)in plant extracts of the Lamiaceae and Oleaceae family. The combination of H-1-C-13 HSQC and H-1-C-13 HMBC techniques allows the connection of the proton and carbon-13 spins across the molecular backbone resulting in the identification and, thus, discrimination of oleanolic and ursolic acid without resorting to physicochemical separation of the components. The quantitative results provided by 2D H-1-C-13 HSQC NMR data were obtained within a short period of time (similar to 14 min) and are in excellent agreement with those obtained by HPLC, which support the efficiency of the suggested methodology. (C) 2009 Elsevier B.V. All rights reserved.
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