期刊
ANALYTICA CHIMICA ACTA
卷 633, 期 1, 页码 97-102出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2008.11.031
关键词
Supramolecular assembly; Fluorescence sensor; Oligonucleotide ligand; Molecular interaction
资金
- NSF of China [20775082, 90717119]
- 973 Program [2007CB935601]
- 863 Program [2007AA02Z221]
- Center for Molecular Science, Institute of Chemistry, CAS
This paper describes a universal, label-free fluorescence sensor that gauges the interaction of oligonucleotides with their targets. The sensor is based on supramolecular assemblies formed by oligonucleotides (polyanions) and small molecule cation surfactant in aqueous solution. The environmentally dependent dye pyrene, encapsulated in the apolar interiors of the assemblies via hydrophobic interactions works as the fluorescence probe. Target binding causes the conformational change of the oligonucleotides, which results in disorganization of supramolecular assemblies, release of pyrene into the aqueous solution and subsequent quenching of its fluorescence. The kinetic processes (including the formation of supramolecular assemblies and the release of pyrenes after adding the targets) were investigated. The fluorescence decreases of pyrenes are proportional to the concentrations of targets within the linear ranges. This label-free fluorescence system is simple,convenient, low cost, and can serve as an alternative tool for interaction studies of oligonucleotides with their targets, especially with small molecular targets. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据