期刊
CHINESE JOURNAL OF CHEMISTRY
卷 25, 期 9, 页码 1233-1237出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cjoc.200790229
关键词
Taq DNA polymerase; colloidal gold; conjugate; biocatalytic activity; polymerase chain reaction
The interaction of colloidal gold with Taq DNA polymerase (Taq) was investigated in this study. Taq-gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV-Vis spectroscopy, X-ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq-gold conjugate particles were still spherical and well-dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据