期刊
ANALYTICA CHIMICA ACTA
卷 630, 期 1, 页码 19-27出版社
ELSEVIER
DOI: 10.1016/j.aca.2008.09.060
关键词
Bisphenol A; Urine; Coacervative microextraction; Human exposure; Liquid chromatography; Fluorescence
资金
- Andalusian Government Gunta de Andalucia, Spain [FQM 166]
- Spanish MEC [AP 2003-5344]
Total (free plus conjugated) urinary BPA is currently being used to assess human exposure to this contaminant. This work proposes the use of coacervates made up of reverse micelles of decanoic acid for the microextraction of BPA, prior to its determination by liquid chromatography and fluorescence detection (lambda(ex) = 276 nm and lambda(em) = 306 nm), with the aim of simplifying sample treatment and reducing analysis time and costs in epidemiologic studies. The procedure involves the enzymatic hydrolysis of 7 mL of urine and then the addition of decanoic acid (100 mg) and tetrahydrofurane (1 mL), conditions under which the coacervate (extractant phase, similar to 167 mu L) forms in situ and instantaneously. The overall procedure takes about 20 min and several samples can be simultaneously treated using conventional lab equipment (i.e. stirrers and centrifuges). Extractions were independent of the pH and temperature in the ranges studied (1-4 and 25-50 degrees C) rendering the method robust. Recoveries in samples ranged between 88 and 95% and the practical detection limit was 0.197 mu g L-1, which is below the usual concentrations of BPA in urine (ranges reported 0.4-149 mu g L-1). The actual concentration factor provided by the method was 38. The precision of the method, expressed as relative standard deviation, was 4.5%. The method was successfully applied to the determination of total BPA in urine from eight healthy volunteers. BPA was detected in all the samples at concentrations ranging between 4.03 and 49 mu g L-1. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据