期刊
JOURNAL OF GENE MEDICINE
卷 9, 期 9, 页码 751-763出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/jgm.1080
关键词
RNA interference; gene silencing; gene therapy; short interfering RNA; short hairpin RNA
资金
- NHLBI NIH HHS [R01 HL052146, R01 HL071628, R01 HL083188] Funding Source: Medline
The application of RNA interference (RNAi) as a research and therapeutic tool depends on its ability to silence genes in a sequence-specific manner. Recent studies have reported that the effective knockdown of genes can be achieved by multiple short hairpin RNAs (shRNAs) in a single vector. Moreover, this approach can depress several genes simultaneously. However, current methods for the construction of multiple shRNA vectors often suffer from vector instability and are time-consuming. Here, we describe a simple, quick and low-cost approach to construct a single vector expressing four shRNA sequences driven by four different promoters. Using this vector, we were able to improve the gene silencing efficiency and make it possible to silence four different genes simultaneously, further expanding the application spectrum of RNAi, both in functional studies and therapeutic strategies. Copyright (c)@ 2007 John Wiley & Sons, Ltd.
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