期刊
ANALYTICA CHIMICA ACTA
卷 610, 期 2, 页码 274-281出版社
ELSEVIER
DOI: 10.1016/j.aca.2008.01.045
关键词
coconut water; phytohormones; high-performance liquid chromatography; liquid chromatography-tandem mass spectrometry
Coconut (Cocos nucifera L.) water, which contains many uncharacterized phytohormones is extensively used as a growth promoting supplement in plant tissue culture. In this paper, a high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of various classes phytohormones, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N-6-benzyladenine (BA), alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) in young coconut water (CW). The analysis was carried out using a reverse-phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid, pH adjusted to 3.2 with triethylamine (TEA)) modified by methanol, and solute detection made at 265 nm. wavelength. The method was validated for specificity; quantification, accuracy and precision. After preconcentration of putative endogenous phytohormones in CW using C-18 solid-phase extraction (SPE) cartridges, the HPLC method was able to screen for putative endogenous phytohormones present in CW Finally, the identities of the putative phytohormones present in CW were further confirmed using independent liquid chromatography-tandem mass spectrometry (LC-MS/MS) equipped with an electrospray ionization (ESI) interface. (c) 2008 Elsevier B.V All rights reserved.
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