期刊
EXPERIMENTAL PARASITOLOGY
卷 117, 期 1, 页码 111-114出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2007.05.011
关键词
Trypanosoma brucei; tsetse; Glossina; GFP; transmission; midgut infection; tetracycline induction
类别
Using green fluorescent protein as a reporter, we have shown that the strain 29-13 of Trypanosoma brucei, widely used for inducible down-regulation of mRNA, is inducible in, but not permissive for the tsetse flies Glossina palpalis gambiensis and Glossina morsitans morsitans. Within two weeks post-infection, 42% males and females of teneral and non-teneral tsetse flies harboured intestinal infections, yet not a single infection progressed into the salivary glands. (c) 2007 Elsevier Inc. All rights reserved.
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