期刊
PLANT CELL AND ENVIRONMENT
卷 30, 期 9, 页码 1035-1040出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-3040.2007.01710.x
关键词
A/C-i curves; mesophyll conductance; photosynthesis model
Photosynthetic responses to carbon dioxide concentration can provide data on a number of important parameters related to leaf physiology. Methods for fitting a model to such data are briefly described. The method will fit the following parameters: V-cmax, J, TPU, R-d and g(m) [maximum carboxylation rate allowed by ribulose 1.5-bisphosphate carboxylase/oxygenase (Rubisco), rate of photosynthetic electron transport (based on NADPH requirement), triose phosphate use, day respiration and mesophyll conductance, respectively]. The method requires at least five data pairs of net CO2 assimilation (A) and [CO2] in the intercellular airspaces of the leaf (Ci) and requires users to indicate the presumed limiting factor. The output is (1) calculated CO2 partial pressure at the sites of carboxylation, C-c, (2) values for the five parameters at the measurement temperature and (3) values adjusted to 25 degrees C to facilitate comparisons. Fitting this model is a way of exploring leaf level photosynthesis. However, interpreting leaf level photosynthesis in terms of underlying biochemistry and biophysics is subject to assumptions that hold to a greater or lesser degree, a major assumption being that all parts of the leaf are behaving in the same way at each instant.
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