期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
卷 1769, 期 9-10, 页码 525-531出版社
ELSEVIER
DOI: 10.1016/j.bbaexp.2007.07.003
关键词
activator protein-1 (AP-1); Chromatin immunoprecipitation (ChIP); matrix metalloprotemase-13 (MM-13); NAPSTER; transactivation; Y-box binding; protein-1 (YB-1)
资金
- NCI NIH HHS [R29 CA073783-05S4, CA73783, R29 CA073783-05, R29 CA073783] Funding Source: Medline
Matrix metalloprotemases (MMPs) are key enzymes that implement degradation of the extracellular matrix during cellular invasion in development, tissue remodeling, and pathogenic disease states. MMP-13 has pivotal roles in the pathogenesis of invasive cancers and arthritis. Here we report the identification of Y-box binding protein-1 (YB-1) as a new repressor of MMP-13 transactivation. YB-1 binds in vitro in DNA affinity chromatography to the activator protein-1 (AP-1) DNA sequence within the MMP-13 promoter. Chromatin immumoprecipitation assays reveal that YB-1 binds in living cells to the MMP-13 gene promoter to a region of the MMP-13 promoter containing the AP-1 site. YB-1 represses tumor promoter-induced MMP-13 promoter transactivation at the AP-1 site. This is the first report demonstrating YB-1 binding in vitro and in living cells to a mammalian AP-1 target gene, and the first report of YB-1 regulation of the MMP-13 promoter. (c) 2007 Elsevier B.V All rights reserved.
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