期刊
ARCHIVES OF ORAL BIOLOGY
卷 52, 期 9, 页码 814-821出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2007.01.015
关键词
dentin; TGF-beta 2; enamel; osteocalcin; AFM; sex-linked
资金
- NIDCR NIH HHS [P60 DE013058-010005, P01 DE009859, P01 DE009859-150011, P01DE09859, P60 DE013058, R03 DE016868-01A1, R03 DE016868] Funding Source: Medline
Initiation of enamel formation requires reciprocal signaling between epithelially and mesenchymally derived cells. Objective: in this study, we used a transgenic mouse model which drives overexpression of an activated form of TGF-beta 2 under control of the osteocalcin promoter, to investigate the role of TGF-beta 2 in the dental mesenchyme, on enamel formation. Design: Dentin and enamel were imaged by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Dentin mechanical properties were characterized for hardness and elasticity, following nanoindentation with a modified AFM. Pores found in enamel were quantified and compared using image analysis software (Scion Image (TM)). Results: The elastic modulus of dentin was significantly reduced in the male TGF-beta 2 overexpressor mice as compared to male wildtype mice, with no significant differences between female mice. Similarly, there were significantly more pores in enamel of the male transgenic mice as compared to male wildtype mice, with no significant differences between female mice. in situ hybridization of the continuously erupting incisor confirmed that osteocalcin expression was limited to the odontoblast cell layer at all stages of tooth formation. Conclusion: TGF-beta 2 overexpression in the dentin matrix, results in sex-linked differences in dentin and enamel formation. (C) 2007 Elsevier Ltd. All rights reserved.
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