期刊
NEUROSCIENCE RESEARCH
卷 59, 期 1, 页码 101-106出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2007.05.014
关键词
planarian; tryptophan hydroxylase; serotonergic neurons; neural network; HPLC; RNA interference
We identified a full-length tryptophan hydroxylase (TPH) gene of planarian Dugesia japonica from a head EST database, and named it DjTPH. Based on whole-mount in situ hybridization and immunofluorescence analyses, DjTPH mRNA and protein were mainly expressed in the nervous system, especially ventral nerve cords and eye pigment cells. Furthermore, DjTPH immunoreactivity was clearly detected at commissure axonal connections in the ventral nerve cords. 5-HT was significantly decreased in DjTPH-knockdown planarians compared with control animals. These results suggest that DjTPH is required for 5-HT biosynthesis, and DjTPH antibody is a useful marker for serotonergic neurons in planarians. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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