期刊
ARCHIVES OF ORAL BIOLOGY
卷 52, 期 9, 页码 869-875出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2007.02.009
关键词
nidus vespae; streptococcus mutans; glucosyltransferases; biofilm
Nidus Vespae (the honeycomb of Polistes olivaceous, P. japonicus Saussure and Parapolybiauaria fabricius) have been extensively used in traditional Chinese medicine, given their multiple pharmacological activities, including antimicrobial, anti-inflammatory, anti-virus, antitumor and anesthetic properties. The present study evaluated the anti - glucosyltransferases (GTFs) activity, anti-adherence and anti-biofilm properties of 95% ethanol/water extract, cyclohexane/ethyl acetate, petroleum ether/ethyl acetate and chloroform/methanol fractions of Nidus Vespae. Chloroform/methanol fraction showed a remarkable capacity for inhibiting the adherence of streptococcus mutans ATCC 25175 to saliva-coated hydroxyapatite disc (S-HA) at sub-MC concentrations. In addition, the Nidus Vespue extract and chemical fractions significantly inhibited the activity of cell-associated and extracellular GTFs at sub-MIC concentrations, and the chloroform/methanol fraction was the most effective one. For the anti-biofilm activity assays, minimum biofilm inhibition concentrations (MBIC50) and minimum biofilm reduction concentrations (MBRC50) were determined using the micro-dilution method. The chloroform/methanol fraction showed the highest anti-biofilm activities with a MBIC50 of 8 mg/ml and a MBRC50 of 16 mg/ml against Streptococcus mutans ATCC 25175. The significant inhibition of GTFs activity and biofilm formation demonstrated by Nidus Vespae shows it to be a promising natural product for the prevention of dental caries. (C) 2007 Elsevier Ltd. All rights reserved.
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