期刊
ANALYST
卷 139, 期 20, 页码 5086-5092出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an00758a
关键词
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资金
- National Natural Science Foundation of China [91023007, 20773033]
- New Century Excellent Talents in University
- Outstanding Young Funding of Heilongjiang Province
- Fundamental Research Funds for the Central Universities [HIT. ICRST. 2010 004]
A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell tine (HepG(2)) within the concentration range of 10 to 10(5) cells per mL. The detection limit for HepG(2) cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.
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