期刊
NATURE BIOTECHNOLOGY
卷 25, 期 9, 页码 1025-1034出版社
NATURE PORTFOLIO
DOI: 10.1038/nbt1334
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资金
- NIAMS NIH HHS [R01 AR 049684, 1U54 AR 050733-01] Funding Source: Medline
- NIDCR NIH HHS [R01 DE 13420-06] Funding Source: Medline
We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56(+) myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56(+) myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease.
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