期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 856, 期 1-2, 页码 273-277出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2007.06.009
关键词
cloud-point extraction; arbidol; HPLC-UV; triton X-114; pharmacokinetics
A method based on cloud-point extraction (CPE) was developed to determine arbidol in rat plasma by high performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as the extract solvent. Variable parameters affecting the CPE efficiency were evaluated and optimized. A Zorbax SB-C-18 column (4.6 mm i.d. x 150 mm, 5 mu m particle size) was used for isocratic elution separation at 40 degrees C with detection wavelength at 316 urn. Under the optimum conditions, the method was shown to be reproducible and reliable with intraday precision below 6.6%, interday precision below 8.8%, accuracy within +/-5.0% and mean extraction recovery more than 89.7%, which were all calculated using a range of spiked samples at three concentrations of 0.2, 2 and 16 mu g/ml for arbidol in plasma. The linear range was from 0.08 to 20 mu g/ml. After strict validation, the method was successfully applied to the pharmacokinetic study of arbidol in rats after oral and intravenous administration, respectively. (C) 2007 Elsevier B.V. All rights reserved.
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