期刊
ANALYST
卷 139, 期 7, 页码 1622-1627出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an01639k
关键词
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资金
- Korea Research Foundation [NRF-2012R1A1A2020074, NRF-2010-0020840]
- KRIBB initiative programs
Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5'-end. The newly designed OTA aptasensor showed enhanced stability and sensitivity, thereby lowering the detection limit to an similar to 1 nM level. Furthermore, an optimal spacer for hemin conjugation was investigated for stable responses toward very diluted OTA solutions.
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