期刊
DEVELOPMENT
卷 134, 期 17, 页码 3121-3131出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.006635
关键词
planarian; gap junctional communication; stem cell; regeneration
资金
- NCRR NIH HHS [C06 RR11244] Funding Source: Medline
- NICHD NIH HHS [R21 HD055850] Funding Source: Medline
- NIGMS NIH HHS [F32 GM078774-01A1, F32 GM078774, F32 GM078774-02] Funding Source: Medline
The largely unknown mechanisms that regulate adult stem cells probably involve signals from neighboring differentiated cells. Gap junction channels providing direct cell-cell communication via small molecules are a crucial component of morphogenesis and normal physiology. However, no specific gap junction protein has yet been functionally linked to adult/somatic stem cell behavior in vivo or to organ regeneration. We report the identification and characterization of smedinx-11 - an innexin gap junction channel gene expressed in the adult stem cells (neoblasts) of the planarian Schmidtea mediterranea. smedinx-11 RNAi treatment inhibits regeneration and abrogates neoblast maintenance. Moreover, smedinx-11 expression is enriched in an irradiation-sensitive subpopulation('X2') and is required for proper expression of other stem cell-specific markers. Analyses of the smedinx-11 downregulation phenotype revealed a striking anterior-posterior neoblast gradient. Our data demonstrate a novel role for gap junction proteins and suggest gap junction-mediated signaling as a new and tractable control point for adult, somatic stem cell regulation.
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