期刊
ANALYST
卷 138, 期 11, 页码 3297-3302出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an36907b
关键词
-
资金
- National Basic Research Program of China (973) [2010CB933603]
- National Natural Science Foundation of China [20935003, 20820102037]
Determining and monitoring toxicity of chemicals in water are very important for human health and country security. Electrochemical measurement of respiratory chain activity is a rapid and reliable screening of the toxicity towards microorganisms. Here, we report a rapid and sensitive toxicity bioassay using p-benzoquinone as the artificial electron mediator and Escherichia coli as the test organism. Four heavy metal ions, Cu2+, Ag+, Hg2+ and Co2+, are tested as the model toxicants, and the corresponding 50% respiration inhibition concentrations (IC50) are determined to be 0.95, 8.14, 11.69 and 42.76 mg L-1, respectively. Based on the IC50 values, the descending order of toxicity is: Cu2+ > Ag+ > Hg2+ > Co2+. The presented bioassay not only provides a good foundation for further toxicity tests using E. coli, but also the potential for expanding the technique to utilize other bacteria with complementary toxicity responses, thereby allowing use of the bioassay in a wide range of applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据