4.6 Article

Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite

期刊

ANALYST
卷 138, 期 18, 页码 5365-5370

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an01006f

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资金

  1. National Science Foundation of China [21271127, 61171033]
  2. NanoFoundation of Science and Techniques Commission of Shanghai Municipality [12nm0504200]
  3. Leading Academic Discipline Project of Shanghai Municipal Education Commission [J50102]

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A novel protocol of electrochemical thrombin aptasensor based on a gold nanoparticles/thionine-graphene (AuNPs/Th-G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine via pi-stacking interaction, and then AuNPs were electrodeposited onto the Th-G surface. The morphology and conductivity of the AuNPs/Th-G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th-G nanocomposite to prepare an electrochemical biosensing platform. The aptamer-thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3).

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