期刊
ANALYST
卷 138, 期 7, 页码 2057-2060出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an36496h
关键词
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资金
- National Natural Science Foundation [21104030, 21073221]
- Shandong Province Higher Educational Science and Technology Program [J11LB03]
We report a label-free fluorescence turn-on approach for the sensitive and selective sensing of silver ion Ag+ based on Ag+ induced conformational change of cytosine-rich single-stranded DNA. A silver specific oligonucleotide (SSO) undergoes a structural transition from single-stranded DNA (ssDNA) to a C-Ag+-C mediated hairpin structure, which can be recognized by a double-stranded DNA (dsDNA) intercalator Sybr Green I (SG). The linear response range for Ag+ detection was from 1 nM to 100 nM. The method presented here is highly sensitive and a limit of detection of 1 nM was obtained. More importantly, this optical method was successfully used to identify complex sample mixtures.
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