An electrochemical biosensor for the direct detection of oxytetracycline in mouse blood serum and urine

Oxytetracycline (OTC), a broad-spectrum antibiotic, has been extensively used as a food additive for livestock. Its extensive use has greatly increased the risk of chronic drug abuse and has also increased the risk of the resulting diseases. Therefore, in light of this emerging situation, the detection of OTC in both food and livestock is very important to reduce the risks and for diagnosis purposes. In this work, we have proposed an electrochemical aptasensor to quantify OTC. The biosensor shows considerable sensitivity and selectivity, and it can be easily operated and regenerated. Furthermore, for the first time, we have shown that an electrochemical aptasensor can be directly used to detect OTC in mouse blood serum and urine. This biosensor has the potential to aid in the analysis of residual OTC levels, as well as providing more pharmacokinetic information in the future.