期刊
ANALYST
卷 138, 期 8, 页码 2350-2356出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an00029j
关键词
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资金
- Key Project of the Natural Science Foundation of China [90606003, 21175039, 21190044]
- International Science & Technology Cooperation Program of China [2010DFB30300]
- Hunan Provincial Natural Science Foundation
- Hunan Provincial Science and Technology Plan of China [10JJ7002, 2011FJ2001]
- Hunan Provincial Graduate research and innovation projects [CX2011B132]
We present here a highly selective and sensitive label-free method to detect Hg2+ ions in aqueous solution by using DNA molecular machine-based fluorescent Ag nanoclusters (AgNCs). This mechanism is based on the Hg2+ ions triggering machine-like operations of DNA and the product of the machine being used to stabilize fluorescent AgNCs. In this method, a tailored DNA, containing a sequence for Hg2+ ions recognition, a sequence-specific nicking site for Nb BbvC I and a sequence complementary to the DNA as a template for the synthesis of fluorescent AgNCs, was firstly designed. In the presence of Hg2+ ions, the machine's function operations were triggered. A series of machine-like operations, including replication, scission, and displacement then occurred with the addition of polymerase/dNTPs/Nb BbvC I, which manufactured lots of product DNA. The product DNA could act as a template for the preparation of fluorescent AgNCs. Thus the fluorescence of the AgNCs could be used as a signal transduction of this DNA machine, which was related to the concentration of the Hg2+ ions. The repeated synthesis of the product and its template effect for AgNCs synthesis led to signal amplification in the assay of Hg2+ ions. A linear response to the concentration of Hg2+ ions was observed in the range from 0.08 nM to 20 nM and a detection limit of 0.08 nM was obtained. By contrast, the operation of the machine could not be executed in an Hg2+ ion-free system. Moreover, the detection was not only label-free but also specific for Hg2+ ions without being affected by other metal ions.
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