期刊
ANALYST
卷 138, 期 9, 页码 2755-2760出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an00122a
关键词
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资金
- National Natural Foundation of China [21205143, 21135001, 91117006]
- Natural Science Foundation of Hunan Province [llJJ5012]
In this paper, the development is described of an efficient pyrene excimer signaling-based fluorescent sensor for the measurement of mercury ions in aqueous solutions based on thymine-Hg2+-thymine (T-Hg2+-T) coordination chemistry and the inclusion interaction of gamma-cyclodextrin. Introduction of cyclodextrin can provide cooperation for the molecular level space proximity of the two labeled pyrene molecules, moreover the hydrophobic cavity of gamma-cyclodextrin can also offer protection for the pyrene dimer's emission from the quenching effect of Hg2+ and enhance the fluorescence intensity of the pyrene excimer. To demonstrate the feasibility of the design, a bis-pyrene-labeled thymine-rich DNA strand was used as the detection probe. In the presence of Hg2+, stem-close-shaped DNA strands can be formed with the cooperation of gamma-cyclodextrin and ideally predominantly emit the excimer fluorescence. The selectivity of the sensor for Hg2+ against other biologically and environmentally related metal ions is outstanding due to the high specificity of T-Hg2+-T formation. In addition, the pyrene excimer has a long fluorescence lifetime, which can tolerate intense background fluorescence interference from complex biological components, making it potentially applicable in the analysis of complex biological samples.
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