期刊
ANALYST
卷 138, 期 3, 页码 760-766出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an36374g
关键词
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资金
- National Basic Research Program of China (973 Program) [2011CB911003]
- National Natural Foundation of China [20975050, 21175066]
- National Science Funds for Creative Research Groups [21121091]
A novel, enzyme-free and aptamer-based colorimetric platform for protein detection has been developed, which takes advantage of aptamer-functionalized magnetic beads (MBs) for target capture, concentration and separation, and aptamer-conjugated gold nanoparticle (AuNP)-catalyzed color bleaching reaction of methyl orange (MO) to generate the colorimetric signals. It was demonstrated that the proposed colorimetric sensing strategy enables simple, cost-effective, sensitive and specific thrombin detection without the use of any enhancing solutions and enzymes. Herein, by naked eye observation, we can detect the human thrombin with a detection limit of approximately 320 pM, which can be further decreased to 30 pM with the help of a UV-vis instrument. In addition, this method also works for targets with two or more binding sites.
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