Hansschlegelia plantiphila gen. nov sp nov., a new aerobic restricted facultative methylotrophic bacterium associated with plants

A new genus, Hansschlegelia, and a new species, Hansschlegelia plantiphila, are proposed for three strains of S-1(T)), methanol-utilizing bacteria isolated from lilac buds (strain 1 linden buds (strain S-2) and blue spruce needles (strain S-4), which were selected in winter at -17 degrees C. These bacteria are aerobic, Gram-negative, colorless, non-motile short rods that multiply by binary fission and employ the ribulose bisphosphate (RuBP) and the serine pathways for C, assimilation. The strains have a limited number of growth substrates and use methanol, methylamine, formate, CO2/ ST grows H-2 and glycerol as carbon and energy sources. Only strain I with ethanol and inulin. The strains are neutrophilic and mesophilic, and synthesize phytohormones (auxins and cytokinins) and vitamin B-12. Their major cellular fatty acids are saturated C-16:0, straight-chain, unsaturated C-18:1 omega 7 and cyclopropane C-19 (cyc) acids. The main ubiquinone is ubiquinone-10 (Q-10). The dominant phospholipids are phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol (cardiolipin). The DNA G+C content is 68.5 + 0.2mol%. The strains share almost identical 16S rRNA gene sequences, a high DNA-DNA hybridization value (72 - 86%) and represent a novel lineage of autotrophic methanol-utilizing bacteria within the Alphaproteobacteria. Collectively, these strains comprise a new ST T T genus and species H. plantiphila gen. nov., sp. nov., with strain S-1(T) (VKM B-2347(T), NCIMB 14035) as the type strain. (c) 2007 Elsevier GmbH. All rights reserved.