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DNA ejection from bacteriophage: Towards a general behavior for osmotic-suppression experiments

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EUROPEAN PHYSICAL JOURNAL E
卷 24, 期 1, 页码 9-18

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SPRINGER
DOI: 10.1140/epje/i2007-10205-5

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We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.

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