期刊
HISTOPATHOLOGY
卷 51, 期 3, 页码 305-312出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-2559.2007.02790.x
关键词
cervix; cKIT; HPV; hypercalcaemic; lung; OCT3/4; ovary; p16; small cell carcinoma; TTF-1; WT-1
Aims: Small cell carcinoma of the ovary, hypercalcaemic-type (SCCOH) is morphologically similar to small cell carcinomas from other sites. The aims of this study were to (i) determine if a biomarker panel would distinguish small cell carcinomas of the ovary, cervix (SCCCx) and lung (SCCLu) and (ii) potentially determine the histogenesis of SCCOH. Methods and results: Nine ovarian small cell carcinomas (seven hypercalcaemic type; two pulmonary type), eight SCCCx and 22 SCCLu were immunostained for thyroid transcription factor (TTF)-1, WT-1, p16, cKIT and OCT3/4; a subset of cases were tested for human papillornavirus (HPV). WT-1 was diffusely positive in 6/7 SSCOH versus two of 33 other small cell carcin-omas (P <= 0.001). TTF-1 was diffusely positive in 20/22 SCCLu and 1/8 SCCCx, and negative in all SCCOH. p16 and cKIT demonstrated variable patterns of immunoreactivity in all cases. HPV was identified in 5/6 SCCCx; SCCOH and SCCLu were negative for HPV. Conclusions: Combined staining with WT-1 and TTF-1 will distinguish SCCOH from SCCLu and SCCCx with a sensitivity of 86% and specificity of 97%. HPV is specific for tumours of cervical origin, but p16 immunohistochemistry is not useful for this purpose. The presence of diffuse WT-1 supports a Mullerian origin for SCCOH, whereas the absence of cKIT and OCT3/4 argues against a germ cell origin.
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