期刊
MOLECULAR ONCOLOGY
卷 1, 期 2, 页码 216-225出版社
WILEY
DOI: 10.1016/j.molonc.2007.06.001
关键词
Proteomics; Plasma protein profiling; Low-volume sandwich-immunoassays; Mouse cancer model
类别
资金
- SAIC/NCI [23Xs110A]
- Eastern Consortium for Proteomics Analyses of Mouse Models of Human Cancers [F012S96]
- Michigan Technology Tri-Corridor [GR-687]
- NIDDK [IK08DK078033-01]
- American Gastroenterological Association
- Van Andel Research Institute
Mouse models of human cancers may provide a valuable resource for the discovery of cancer biomarkers. We have developed a practical strategy for profiling specific proteins in mouse plasma using low-volume sandwich-immunoassays. We used this method to profile the levels of 14 different cytokines, acute-phase reactants, and other cancer markers in plasma from mouse models of intestinal tumors and their wild-type littermates, using as little as 1.5 mu l of diluted plasma per assay. many of the proteins were significantly and consistently up-regulated in the mutant mice. The mutant mice could be distinguished nearly perfectly from the wild-type mice based on the combined levels of as few as three markers. Many of the proteins were up-regulated even in the mutant mice with few or no tumors, suggesting the presence of a systemic host response at an early stage of cancer development. These results have implications for the study of host responses in mouse models of cancers and demonstrate the value of a new low-volume, high-throughput sandwich-immunoassay method for sensitively profiling protein levels in cancer. (C) 2007 Federation of European Biochemical Societies Published by Elsevier B.V. All rights reserved
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