期刊
CELL HOST & MICROBE
卷 2, 期 3, 页码 181-192出版社
CELL PRESS
DOI: 10.1016/j.chom.2007.07.003
关键词
-
资金
- NIAID NIH HHS [R01AI5228] Funding Source: Medline
- NIDDK NIH HHS [R01 DK061931-06, R01 DK061931] Funding Source: Medline
The major group B coxsackievirus (CVB) receptor is a component of the epithelial tightjunction (TJ), a protein complex that regulates the selective passage of ions and molecules across the epithelium. CVB enters polarized epithelial cells from the TJ, causing a transient disruption of TJ integrity. Here we show that CVB does not induce major reorganization of the TJ, but stimulates the specific internalization of occludin-a TJ integral membrane component-within macropinosomes. Although occludin does not interact directly with virus, depletion of occludin prevents CVB entry into the cytoplasm and inhibits infection. Both occludin internalization and CVB entry require caveolin but not dynamin; both are blocked by inhibitors of macropinocytosis and require the activity of Rab34 Ras, and Rab5, GTPases known to regulate macropinocytosis. Thus, CVB entry depends on occludin and occurs by a process that combines aspects of caveolar endocytosis with features characteristic of macropinocytosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据