期刊
ANALYST
卷 135, 期 5, 页码 1131-1137出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b921409g
关键词
-
资金
- Office of Naval Research through the Hawaii Environmental and Energy Technology (HEET) initiative (Richard Rocheleau, PI) [N0014-01-1-0928]
- AFOSR Multidisciplinary University Research Initiative program [FA9550-06-1-0264]
In this work the shift in fluorescence emission spectra of acrylodan, a polar sensitive fluorophore, has been used to characterize the polarity immediately surrounding cytoplasmic (cMDH) and mitochondrial malate dehydrogenase (mMDH) enzyme immobilized within three-dimensional macroporous chitosan scaffolds. The scaffolds were fabricated from solutions of fluorescently tagged enzymes mixed with deacetylated and hydrophobically modified chitosan polymer. Each solution was frozen and then freeze-dried through the process of thermally induced phase separation (TIPS). The blue shift in acrylodan's emission maxima (lambda(max)) revealed a polar shift in the chemical microenvironment surrounding the enzymes when immobilized in a modified as opposed to unmodified chitosan scaffold. These results suggest that the method of hydrophobic modification of native chitosan polymer can be used to control the amphiphilic nature of the chemical microenvironment immediately surrounding the enzyme after it has been immobilized.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据