期刊
ANALYST
卷 135, 期 7, 页码 1779-1784出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00168f
关键词
-
资金
- NSFC
- Chinese Academy of Sciences
- NSFC-DFG [TRR61]
As essential enzymes in the body, monoamine oxidases (MAOs), including MAO-B, help maintain the proper balance of neurotransmitters and other dietary and biogenic amines. Thus, development of convenient and continuous assays for MAO-B and its inhibitor-screening is highly desirable since the inhibitors have implications for therapeutics for many brain diseases. In this paper, we report a direct continuous fluorescence turn-on assay for MAO-B and its inhibitor-screening with the ensemble of silole 1, heptylamine and HSO(3)(-), which are either easily accessible or commercially available. Fluorescent spectral investigations show that this fluorometric assay can be performed continuously in aqueous solution and MAO-B of concentrations as low as 0.25 mu g/mL can be analyzed. Moreover, the ensemble of silole 1, heptylamine, HSO(3)(-) and MAO-B can be employed to screen the inhibitors of MAO-B.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据