期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 55, 期 18, 页码 7258-7263出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf070483r
关键词
pyrroloquinoline quinone (PQQ); extraction; quantification; LC/MS/MS analysis
Pyrroloquinoline quinone (PQQ) is believed to be an important factor for mammalian growth and development and has, therefore, been declared a vitamin by some researchers. However, this issue remains controversial, and from a nutritional viewpoint, accurate determination of PQQ levels in a variety of foods is very important. Here, we describe a simple, highly sensitive, and highly selective method for quantitative analysis of PQQ. Liquid foods or aqueous extracts of solid foods were analyzed using high-performance liquid chromatography (HPLC) combined with electrospray-ionization (ESI) tandem mass spectrometry (MS/MS). N-15-labeled POO was added to the samples as an internal standard. Quantitative analyses of POO were performed by multiple reaction monitoring (MRM) with LC/MS/MS. Free PQO was detected in almost all food samples in the range 0.19-7.02 ng per g fresh weight (for solid foods) or per mL (liquid foods). This method will enable the rapid and simple determination of POO levels in many samples.
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