期刊
ANALYST
卷 133, 期 9, 页码 1182-1186出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b805334k
关键词
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资金
- Ministry of Science & Technology of China [2006BAK02A09, 2006CB933000, 2007AA06A406, 2007CB936000]
- Shanghai Leading Academic Discipline Project [13205]
- National Natural Science Foundation [60537030, 20725516]
- Shanghai Municipal Commission for Science and Technology [0652nm006, 0752nm021]
There has been urgent demand for rapid, sensitive and cost-effective pesticide assay technologies due to the global attention of environmental and food-safety problems. Acetycholinesterase (AChE)-based electrochemical sensors have attracted significant interest toward this goal. In this contribution, we introduced multiwalled carbon nanotubes (MWNTs) into our sensor design, where they played dual enhancement roles; first is that MWNTs loaded on glassy carbon (GQ electrodes significantly increase surface areas, facilitating the electrochemical polymerization of prussian blue (PB), a redox mediator for the electrochemical oxidation of the enzymatic product, thiocholine (TCh). Second, MWNTs enhance the enzymatic activity of AChE, as manifested by the decreased Michaelis-Menten constant (K-m). As a result of these two important enhancement factors offered by MWNTs, our electrochemical pesticide sensor exhibited rapid response and high sensitivity toward the detection of a series of pesticides. Moreover, we demonstrated that this sensor was stable, reproducible and selective enough for detection in real samples.
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