期刊
EXPERIMENTAL CELL RESEARCH
卷 313, 期 15, 页码 3308-3318出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2007.05.031
关键词
sphingosine kinase 1; fibroblast growth factor 1; copper; non-classical release; angiogenesis
资金
- NCRR NIH HHS [P20 RR015555, RR15555, P20 RR015555-076466] Funding Source: Medline
- NHLBI NIH HHS [HL 35627, R01 HL032348-22, HL67330, R01 HL032348, R01 HL035627, HL60694, HL32348, R37 HL067330, R01 HL035627-21, R01 HL067330] Funding Source: Medline
Sphingosine kinase 1 catalyzes the formation of sphingosine-1-phosphate, a lipid mediator involved in the regulation of angiogenesis. Sphingosine kinase I is constitutively released from cells, even though it lacks a classical signal peptide sequence. Because copper-dependent non-classical stress-induced release of FGF1 also regulates angiogenesis, we questioned whether sphingosine kinase 1 is involved in the FGF1 release pathway. We report that (i) the coexpression of sphingosine kinase 1 with FGF1 inhibited the release of sphingosine kinase 1 at 37 degrees C; (ii) sphingosine kinase 1 was released at 42 degrees C in complex with FGF1; (iii) sphingosine kinase 1 null cells failed to release FGF1 at stress; (iv) sphingosine kinase 1 is a high affinity copper-binding protein which formed a complex with FGF1 in a cell-free system, and (v) sphingosine kinase 1 over expression rescued the release of FGF1 from inhibition by the copper chelator, tetrathiomolybdate. We propose that sphingosine kinase 1 is a component of the copper-dependent FGF1 release pathway. (c) 2007 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据