期刊
JOURNAL OF IMMUNOLOGY
卷 179, 期 6, 页码 3707-3714出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.179.6.3707
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- NIAID NIH HHS [R01 AI054624] Funding Source: Medline
Ligation of the purinergic receptor, P2X(7)R, with its agonist ATP has been previously shown to inhibit intracellular infection by chlamydiae and mycobacteria in macrophages. The effect of P2X(7)R on chlamydial infection had never been investigated in the preferred target cells of chlamydiae, cervical epithelial cells, nor in vaginally infected mice. In this study, we show that treatment of epithelial cells with P2X(7)R agonists inhibits partially Chlamydia infection in epithelial cells. Chelation of ATP with magnesium or pretreatment with a P2X7R antagonist blocks the inhibitory effects of ATP. Similarly to previous results obtained with macrophages, ATP-mediated inhibition of infection in epithelial cells requires activation of host-cell phospholipase D. Vaginal infection was also more efficient in P2X(7)R-deficient mice, which also displayed a higher level of acute inflammation in the endocervix, oviduct, and mesosalpingeal tissues than in infected wild-type mice. However, secretion of IL-1 beta, which requires P2X(7)R ligation during infection by other pathogens, was decreased mildly and only at short times of infection. Taken together, these results suggest that P2X(7)R affects Chlamydia infection by directly inhibiting infection in epithelial cells, rather of P2X(7)R to modulate IL-1 beta secretion.
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