期刊
JOURNAL OF CELL SCIENCE
卷 120, 期 18, 页码 3262-3270出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.012328
关键词
JAK/STAT signalling; MHC; chromatin architecture; decondensation; looping
类别
资金
- Cancer Research UK [A8318] Funding Source: Medline
Transcriptional activation of the major histocompatibility complex (MHC) by IFN gamma is a key step in cell-mediated immunity. At an early stage of IFN gamma induction, chromatin carrying the entire MHC locus loops out from the chromosome 6 territory. We show here that JAK/STAT signalling triggers this higher-order chromatin remodelling and the entire MHC locus becomes decondensed prior to transcriptional activation of the classical HLA class II genes. A single point mutation of STAT1 that prevents phosphorylation is sufficient to abolish chromatin remodelling, thus establishing a direct link between the JAK/STAT signalling pathway and human chromatin architecture. The onset of chromatin remodelling corresponds with the binding of activated STAT1 and the chromatin remodelling enzyme BRG1 at specific sites within the MHC, and is followed by RNA-polymerase recruitment and histone hyperacetylation. We propose that the higher-order chromatin remodelling of the MHC locus is an essential step to generate a transcriptionally permissive chromatin environment for subsequent activation of classical HLA genes.
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