期刊
GENES & DEVELOPMENT
卷 21, 期 18, 页码 2271-2276出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1586107
关键词
rational design; synthetic biology; positive feedback; memory; in vivo quantitation
资金
- NCI NIH HHS [T32 CA09361, T32 CA009361] Funding Source: Medline
The ability to logically engineer novel cellular functions promises a deeper understanding of biological systems. Here we demonstrate the rational design of cellular memory in yeast that employs autoregulatory transcriptional positive feedback. We built a set of transcriptional activators and quantitatively characterized their effects on gene expression in living cells. Modeling in conjunction with the quantitative characterization of the activator-promoter pairs accurately predicts the behavior of the memory network. This study demonstrates the power of taking advantage of components with measured quantitative parameters to specify eukaryotic regulatory networks with desired properties.
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