期刊
DEVELOPMENTAL BIOLOGY
卷 309, 期 2, 页码 358-372出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2007.06.012
关键词
zebrafish; box; Hindbrain; rhombomere 4; branchiornotor neurons; facial neurons; neuronal migration; prickle
资金
- NIGMS NIH HHS [T32 GM07183, T32 GM07839] Funding Source: Medline
Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene plays a critical and conserved role in vertebrate hindbrain development, conferring identity to hindbrain rhombomere (r) 4. For example, zebrafish Hoxb I a, similar to mouse Hoxb 1, is required for the migration of r4-derived facial branchiomotor neurons into the posterior hindbrain. We have screened microarrays carrying more than 16,000 expressed. sequence tags (ESTs) for genes that are differentially regulated in normal versus Hoxb I a-deficient r4 tissue. Using this approach, we have identified both positively and negatively regulated candidate Hoxb1a target genes. We have used in situ hybridization to validate twelve positively regulated Hoxb1a targets. These downstream targets are expressed in a variety of subdomains within r4, with one gene, a novel prickle homolog (pk1b), expressed specifically within the facial branchiomotor neurons. Using morpholino knock-down and cell transplantation, we demonstrate that the Hoxb 1a target Prickle 1b functions cell-autonomously to control facial neuron migration, a single aspect of r4 identity. (C) 2007 Elsevier Inc. All rights reserved.
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