期刊
FEBS LETTERS
卷 581, 期 23, 页码 4439-4442出版社
WILEY
DOI: 10.1016/j.febslet.2007.08.019
关键词
amphitropic protein; bacterial cell cycle; macromolecular crowding; membrane occupancy; protein-membrane interaction
DnaA(L366K), in concert with a wild-type DnaA (wtDnaA) protein, restores the growth of Escherichia coli cells arrested in the absence of adequate levels of cellular acidic phospholipids. In vitro and in vivo studies showed that DnaA(L366K) alone does not induce the initiation of replication, and wtDnaA must also be present. Hitherto the different behavior of wt and mutant DnaA were not understood. We now demonstrate that this mutant may he activated at significantly lower concentrations of acidic phospholipids than the wild-type protein, and this may explain the observed growth restoration in vivo. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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