期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 372, 期 3, 页码 660-671出版社
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2007.06.082
关键词
poxvirus; vaccinia virus; tumour necrosis factor (TNF); tumour necrosis factor receptor (TNFR); immune modulation
资金
- Medical Research Council [G0500365] Funding Source: researchfish
- Medical Research Council [G0500365] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- MRC [G0500365] Funding Source: UKRI
Vaccinia virus (VACV), the smallpox vaccine, encodes many proteins that subvert the host immune response. One of these, cytokine response modifier E (CrmE), is secreted by infected cells and protects these cells from apoptotic challenge by tumour necrosis factor alpha (TNF alpha). We have expressed recombinant CrmE from VACV strain Lister in Escherichia coli, shown that the purified protein is monomeric in solution and competent to bind TNF alpha, and solved the structure to 2.0 angstrom resolution. This is the first structure of a virus-encoded tumour necrosis factor receptor (TNFR). CrmE shares significant sequence similarity with mammalian type 2 TNF receptors (TNFSFR1B, p75, TNFR type 2). The structure confirms that CrmE adopts the canonical, TNFR fold but only one of the two ligand-binding loops of TNFRSF1A is conserved in CrmE, suggesting a mechanism for the higher affinity of poxvirus TNFRs for TNF alpha over lymphotoxin-alpha. The roles of dimerisation and pre-ligand-assembly domains (PLADs) in poxvirus and mammalian TNFR activity are discussed.
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