期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 126, 期 2, 页码 354-360出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2007.03.021
关键词
immumosensor; mass change; flow cell; resonance frequency
We report a highly sensitive and rapid method for the detection of Staphylococcus aureus enterotoxin B (SEB) at picogram levels using a piezoelectric -excited millimeter- sized cantilever (PEMC) sensor. Affinity purified polyclonal antibody to Staphylococcal enterotoxin B (anti-SEB) was immobilized on the aminated cantilever glass surface, followed by exposure of SEB containing samples flowing at a rate of 1 mL/min. The binding of SEB was measured by monitoring the sensor's fundamental resonance frequency. The fundamental frequencies in air and under liquid immersion were 17.50 +/- 0.01 kHz and 10.37 +/- 0.01 kHz, respectively. Binding of SEB to the sensor surface resulted in an exponential decrease of the resonance frequency and reached a steady state frequency change of 31 +/- 1 Hz (n = 2) and 208 +/- 1 Hz (n = 2) corresponding to SEB concentrations of 50 pg/mL and 12.5 ng/mL. respectively. In each experiment, the bound SEB was released by a pH 2.0 HCI/PBS solution and the sensor response was nearly identical to the frequency change caused by attachment. Full antibody surface regeneration was obtained after the release by rinsing, the sensor with PBS pH 7.4. We report the detection limit of PEMC sensors to SEB is between 12.5 pg/mL and 50 pg/mL. (C) 2007 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据