期刊
EXPERT REVIEW OF PROTEOMICS
卷 4, 期 5, 页码 627-637出版社
TAYLOR & FRANCIS LTD
DOI: 10.1586/14789450.4.5.627
关键词
2D gel electrophoresis; biostatistics; imniunofluorescence; profiling protein extraction
资金
- NCI NIH HHS [R01 CA107304-04A1, R01 CA107304, CA107304] Funding Source: Medline
- NIAAA NIH HHS [R56 AA013886, AA16157, AA13886, R01 AA016157] Funding Source: Medline
- NIDA NIH HHS [P30 DA018310, DA18310] Funding Source: Medline
Proteomics holds the promise of evaluating global changes in protein expression and post-translational modification in response to environmental stimuli. However, difficulties in achieving cellular anatomic resolution and extracting specific types of proteins from cells have limited the efficacy of these techniques. Laser capture microdissection has provided a solution to the problem of anatomical resolution in tissues. New extraction methodologies have expanded the range of proteins identified in subsequent analyses. This review will examine the application of laser capture microdissection to proteomic tissue sampling, and subsequent extraction of these samples for differential expression analysis. Statistical and other quantitative issues important for the analysis of the highly complex datasets generated are also reviewed.
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