期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 389, 期 3, 页码 931-940出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1493-7
关键词
T-2 toxin; mass spectrometry; isotope dilution; internal standard; maize; oat
In this paper, the structure and the identity of fully C-13-substituted T-2 toxin were confirmed using high-resolution mass spectrometry, H-1-NMR, C-13-NMR, tandem mass spectrometry and HPLC-DAD. The purity of this compound was estimated to be at least 98.8% according to UV data. The isotopic distribution of (C-13(24)) T-2 toxin indicated a total isotopic enrichment of 98.2 +/- 1.0 atom% C-13, and the application of different MS measurement modes revealed the MS/MS fragmentation pattern of T-2 toxin. Furthermore, a stable isotope dilution mass spectrometry method for the quantification of T-2 toxin was developed using (C-13(24)) T-2 toxin as internal standard. The method was evaluated with and without conventional clean-up and validated for maize and oats. Both cereals showed strong matrix enhancement effects, which could be compensated for through the application of the isotope-substituted internal standard.
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