期刊
DEVELOPMENT
卷 134, 期 19, 页码 3527-3537出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.002352
关键词
Ciona; ascidian; neural patterning; Nodal; Delta; Notch; FGF; MEK; ERK
The ascidian neural plate has a grid- like organisation, with six rows and eight columns of aligned cells, generated by a series of stereotypical cell divisions. We have defined unique molecular signatures for each of the eight cells in the posterior- most two rows of the neural plate - rows I and II. Using a combination of morpholino gene knockdown, dominant- negative forms and pharmacological inhibitors, we tested the role of three signalling pathways in defining these distinct cell identities. Nodal signalling at the 64- cell stage was found to be required to define two different neural plate domains - medial and lateral - with Nodal inducing lateral and repressing medial identities. Delta2, an early Nodal target, was found to then subdivide each of the lateral and medial domains to generate four columns. Finally, a separate signalling system along the anteroposterior axis, involving restricted ERK1/ 2 activation, was found to promote row I fates and repress row II fates. Our results reveal how the sequential integration of three signalling pathways - Nodal, Delta2/ Notch and FGF/ MEK/ ERK - defines eight different sub- domains that characterise the ascidian caudal neural plate. Most remarkably, the distinct fates of the eight neural precursors are each determined by a unique combination of inputs from these three signalling pathways.
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